Disc Electrophoresis of Turkey Serum Alkaline Phosphatase Isoenzymes Using Polyacrylamide Gel
نویسندگان
چکیده
منابع مشابه
Canine serum alkaline phosphatase isoenzymes detected by polyacrylamide gel disk electrophoresis.
Serum alkaline phosphatase (ALP) isoenzymes were studied in normal dogs using a commercially available polyacrylamide gel disk electrophoresis kit (PAG/disk kit). Serum samples taken from the dogs were incubated with neuraminidase, after which most showed ALP isoenzymes as two characteristic stained bands. To determine the origin of each band, ALP isoenzymes of serum and tissue extracts (liver,...
متن کاملSerum lipoprotein estimation by polyacrylamide gel disc electrophoresis.
A simple, rapid method is described for separating the serum lipoproteins into clear, discrete and reproducible bands by polyacrylamide disc gel electrophoresis. The sample is prestained with Sudan Black B in a sample gel and resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel and separating gel. 30 young healthy males and 20 young healthy fema...
متن کاملPolyacrylamide gel electrophoresis of isoenzymes from Entamoeba species.
In this preliminary report, we describe a polyacrylamide gel electrophoresis technique for the resolution of isoenzyme patterns of four isolates of Entamoeba histolytica and one isolate of Entamoeba coli. Our findings were similar to previous findings for three enzyme systems: maleic enzyme (malate dehydrogenase [EC 1.1.1.40]), hexokinase (EC 2.7.1.1), and phosphoglucomutase (EC 2.7.5.1). We fo...
متن کاملSerum Alkaline Phosphatase Isoenzymes in Lymphoproliferative Diseases1
A new isoenzyme of alkaline phosphatase (EC 3.1.3.1) has been reported to occur in sera from patients with lymphoproliferative diseases. This enzyme is character ized by an inability to hydrolyze cysteamine S-phosphate. We find that the 5,5 -dithobis(2-nitrobenzoic acid)-coupled assay method for cysteamine S-phosphate hydrolysis is not suitable for serum, and we were unable to confirm the exist...
متن کاملDetection of serum alkaline phosphatase isoenzymes with phenolphthalein monophosphate following cellulose acetate electrophoresis.
Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 mm. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting and scanning procedures. The sensitiv...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Poultry Science
سال: 1975
ISSN: 0032-5791
DOI: 10.3382/ps.0541366